Introduction to Aquatic Flow Cytometry Course

When: April 22-26, 2024

Where: Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine, USA

This 5-days course is organized by Dr. Nicole Poulton, Director of the Center for Aquatic Cytometry and will include additional lecturers who are experts within their fields. It will include both lecture and laboratory sessions, and provides aquatic and environmental scientists and/or commercial entities an introduction to flow cytometry, including the theory of operation as well as an introduction to different flow cytometric instrumentation – analyzers (ZE5 Cell Analyzer – BioRad & Northern Lights Spectral Analyzer – Cytek) and cell sorter(s) (BD Influx) including imaging cytometry (FlowCAM).

Breakfast and lunch are provided each day and the course will also include one group dinner, and lodging at the campus is included with the course fees.

Throughout the week topics will include discussions on the wide number of applications within the aquatic sciences including, but not limited to:

  1. Culture & Environmental sample enumeration (viruses, bacteria, & phytoplankton), including preservation techniques.
  2. Spectral flow cytometry in aquatic applications.
  3. Cell Sorting for Biogeochemical analyses (C, N, & P)
  4. Cell sorting for isolation and cultivation from aquatic and environmental samples (ie. phytoplankton).
  5. Genome to Phenome analysis using single cell genomics.
  6. Imaging Cytometry for aquatic applications (using FlowCAM).

Registration is limited to 10 participants. Registration and information here.

Nicole Poulton, Ph.D. – Director of Aquatic Cytometry – Bigelow Laboratory
Sophie Clayton, Ph.D. – Imaging Flow Cytobot – Imaging Cytometry – NOC
Melody Lindsay, Ph.D. – Genome to Phenome & Single cell genomics – Bigelow Laboratory
Savannah Judge – Imaging Cytometry – Yokogawa, Fluid Imaging Technologies
Laura Lubelczyk – Research Associate, Center for Aquatic Cytometry

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